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- TI SPAWNING SEASON AND MICROHABITAT USE BY CALIFORNIA GOLDEN TROUT
- (ONCORHYNCHUS MYKISS AGUABONITA) IN THE SOUTHERN SIERRA NEVADA
- AU STEFFERUD J A (Reprint).
- SO CALIFORNIA FISH AND GAME, 1993 FAL, v79, n4 p.133-144
- AB Spawning of California golden trout (Oncorhynchus mykiss aguabonita)
- was observed during May and June 1981, in Cottonwood and Mulkey
- creeks in the southern Sierra Nevada, California. Spawning began when
- daily water temperatures consistently exceeded 10-degrees-C, and was
- most intense between 1300 and 1600 h when water temperature was 16 to
- 18-degrees-C. Apparent election of redd site was based on water
- velocity and size of substrate, but not on water depth or distance to
- cover. Water velocity over redds was between 25 and 77 cm/s (mean 49
- cm/s), and substrate in redds was gravel 2.0 to 19 mm in diameter.
- Golden trout constructed redds in water 4 to 24 cm deep. Redds
- averaged 0.22 m2 in surface area, and were within one-fourth of the
- stream width from the stream bank. Distance to cover from a redd
- often exceeded 15 m.
- GA PD133
-
- 2
- TI A COMPARISON OF PATHOLOGICAL CHANGES CAUSED BY VIBRIO ANGUILLARUM AND
- ITS EXTRACELLULAR PRODUCTS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)
- AU LAMAS J (Reprint). SANTOS Y. BRUNO D. TORANZO A E. ANADON R.
- SO FISH PATHOLOGY, 1994 JUN, v29, n2 p.79-89
- AB Some hematological parameters and histopathological changes in the
- liver, spleen, kidney, gills, gut, skin, muscle and heart of rainbow
- trout (Oncorhynchus mykiss) injected intraperitoneally with virulent
- Vibrio anguillarum or with V. anguillarum extracellular products
- (ECPs) were compared.
- The number of circulating red blood cells, the hematocrit and
- hemoglobin values decreased in the fish injected with either bacteria
- or ECPs compared with controls. The erythrocyte sedimentation rate
- increased in both groups of injected fish; however, the serum protein
- levels were similar to those observed in control fish.
- Light and electron microscopic observations of samples obtained from
- fish injected with V. anguillarum cells or its ECPs revealed similar
- histopathological changes in most tissues. Both groups of fish showed
- degeneration and necrosis of hepatocytes, vasodilation of the
- glomerular capillaries, cloudy swelling and hyaline droplet
- degeneration of the epithelial tubular cells, edematous gills,
- desquamation of the intestinal mucosa and necrosis of the muscle
- fibers at the site of injection. However, the bacterium multiplied
- extensively in the kidney and spleen where it caused more acute
- alterations than ECPs.
- Based on these results, it was concluded that the extracellular
- products play an important role in the virulence mechanisms of V.
- anguillarum.
- GA PD145
-
- 3
- TI EXPERIMENTAL TREATMENT WITH INCREASED TEMPERATURE OF JUVENILE COHO
- SALMON WITH EIBS
- AU TANAKA M (Reprint). OKAMOTO N. SUZUKI M. IGARASHI Y.
- TAKAHASHI K. ROHOVEC J S.
- SO FISH PATHOLOGY, 1994 JUN, v29, n2 p.91-94
- AB Experimental treatment by increasing temperature from 10-degrees-C to
- 16-degrees-C was carried out on juvenile coho salmon (Oncorhynchus
- kisutch) naturally infected with erythrocytic inclusion body syndrome
- (EIBS).
- Cumulative mortality of control fish kept at 10-degrees-C throughout
- the experimental period of 40 days was 57%. In contrast, that of
- experimental fish kept at 16-degrees-C for the first 23 days and then
- at 10-degrees-C for 17 days was 10%. The longer the period at which
- the fish were kept at the higher temperature (16-degrees-C), the
- lower the mortality. Experimental challenge with EIBS to the
- survivors of the temperature-increasing treatment revealed an
- increase in the resistance to reinfection of EIBS.
- GA PD145
-
- 4
- TI A STUDY ON SEROTYPING OF CYTOPHAGA PSYCHROPHILA ISOLATED FROM FISHES
- IN JAPAN
- AU WAKABAYASHI H (Reprint). TOYAMA T. IIDA T.
- SO FISH PATHOLOGY, 1994 JUN, v29, n2 p.101-104
- AB The serological characteristics of the strains of Cytophaga
- psychrophila isolated from different fishes in different areas were
- compared by the microtiter method. Included were 3 strains from cobo
- salmon (Oncorhynchus kisutch) in Miyagi Prefecture, 4 from rainbow
- trout (Oncorhynchus mykiss) in Nagano and Tokyo Pref., 6 from ayu
- (Plecoglossus altivelis) in Tokushima and Wakayama Pref. In addition
- to these Japanese strains, 3 strains from coho salmon in USA were
- used as references. Three antisera were prepared with the type strain
- NCMB1947 from coho salmon in USA, FPC828 from coho salmon in Miyagi,
- and FPC840 from ayu in Tokushima. In the comparison of C.
- psychrophila strains by using these antisera, all were positively
- agglutinated by each of the antisera, indicating the presence of
- common antigen(s). On the basis of the absorption analysis with
- thermostable antigens, two distinct serotypes (O-1 and O-2) were
- recognized. The strains from coho salmon in Japan and USA belonged to
- serotype O-1. Serotype O-2 occurred only in Japan, and was
- distributed among rainbow trout as well as ayu.
- GA PD145
-
-